Parviz Parvizi
Pasteur Institute of Iran
Title: Analyses of five nuclear and mitochondrial genes for the identification the old and new world Leishmania parasites
Biography
Biography: Parviz Parvizi
Abstract
Background: The polymorphism and genetic diversity of Leishmania genus have status under discussion depending on many items from clinical samples, reservoir hosts and vectors.
Objectives: The objectives are to compare genetic changes and the role of each two enzymatic and mitochondrial genes (COII and Cyt b) or housekeeping and nuclear genes (nagt, ITS-rDNA and HSP70) for accurate identification of Leishmania parasites.
Methods: After DNA extractions, PCR products were sequenced and evaluation of genetic proximity and phylogenetic analysis were performed using MEGA6 and DnaSP5 software.
Results: Of the 72 sequences, 54 new haplotypes from five Leishmania species were submitted to GenBank. The number of polymorphic sites was significantly lower as compared to the monomorphic sites.
Conclusions: The highest and lowest levels of haplotype diversity were observed in L. tropica (81.35%) and L. major (28.38%). Tajima’s D index analyses showed that Cyt b gene in L. tropica species was significantly negative, while COII and nagt genes were produced through evolutionary processes for both L. tropica and L. major.