Molecular and Clinical Diagnostics in Pathology

Molecular and clinical diagnostics have revolutionized pathology by enabling highly sensitive and specific detection of genetic, infectious, and metabolic disorders. Techniques such as PCR, real-time PCR, digital PCR, and next-generation sequencing (NGS) allow laboratories to identify minute amounts of DNA or RNA, facilitating early and accurate diagnosis. These tools are critical in oncology for detecting gene mutations and chromosomal abnormalities that guide targeted therapies and personalized treatment, and in infectious diseases for rapid pathogen identification when traditional methods are slow or inconclusive. Integration with conventional laboratory approaches, including hematology, clinical chemistry, and immunology, enhances diagnostic accuracy and provides comprehensive patient insights. Emerging technologies such as liquid biopsies, single-cell sequencing, and point-of-care testing offer minimally invasive, real-time monitoring options. Advances in laboratory automation, digital pathology, and AI-assisted image analysis improve workflow efficiency, reduce errors, and allow high-throughput processing. Emphasis on biomarker discovery, assay standardization, quality control, and regulatory compliance ensures reliability across laboratories. By leveraging these innovations, pathologists and laboratory scientists can provide precise, personalized diagnostic information, improving patient outcomes and advancing the field of modern medicine.

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